Sequencing Heparan Sulphate
Novel strategies for sequence analysis of
HS and other glycosaminoglycans
The lab has a strong commitment to innovative research in
the field of
HS structural analysis. This work is continuing (in
collaboration with Prof.
Hopwood (Adelaide W & C Hospital, Australia)
and Dr Ram Sasisekharan (MIT, Boston, USA).
These are important projects on development
of novel strategies for direct and rapid
sequencing of complex HS and GAG saccharides
using lysosomal exoenzymes (Turnbull et al,
1999).
These methods are crucial to to structure/function
studies on HS. Techniques being used include
electrophoresis, MALDI mass spec and capillary
electrophoresis
Drummond, KJ, Yates, EA & Turnbull JE. (2001) Electrophoretic sequencing of heparin/heparan sulfate oligosaccharides using a highly sensitive fluorescent end label. Proteomics 1, 304-310.
Shriver Z, Raman R, Venkataraman G, Drummond K, Turnbull JE, Toida T, Linhardt R, Biemann K, & Sasisekharan R. (2000) Sequencing of 3-O Sulfate Containing Decasaccharides With a Partial Antithrombin III Binding Site. Proc. Nat. Acad. Sci. USA 97, 10359-10364.
Turnbull, JE, Hopwood, J. & Gallagher, JT. (1999) A strategy for rapid sequencing of heparan sulphate/heparin saccharides. Proc. Nat. Acad. Sci. USA 96, 2698-2703
